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→Glycerol gelatine (Kaiser) stained with fuchsine (0.007%)[this staining method starts with slide 09-100]
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=== Microscope, Cameras and Software === | === Microscope, Cameras and Software === | ||
*Light microscope Weso BM Pro1100, magnification usually 400x (Eyepiece WF 10x/Objective 40/0.65x) ->[http://www.weso.ch/start.html Weso] | *Light microscope Weso BM Pro1100, magnification usually 400x (Eyepiece WF 10x/Objective 40/0.65x) ->[http://www.weso.ch/start.html Weso] | ||
[[File:Weso LM.jpg|160px]] | |||
*Moticam 1000 and Moticam 5 MPixel with MoticImages V2.0/Windows 7 Professional 64 ->[http://www.motic.com/index.php Moticam] | *Moticam 1000 and Moticam 5 MPixel with MoticImages V2.0/Windows 7 Professional 64 ->[http://www.motic.com/index.php Moticam] | ||
*For retrieval of interesting structures on the pollen slides, I use a [http://www.pyser-sgi.com/graticules/stage-micrometers-calibration-scales-grids/stage-micrometers-grids-s-series/s7-england-finder-slide-detail Pyser S7 England Finder] | *For retrieval of interesting structures on the pollen slides, I use a [http://www.pyser-sgi.com/graticules/stage-micrometers-calibration-scales-grids/stage-micrometers-grids-s-series/s7-england-finder-slide-detail Pyser S7 England Finder] | ||
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=== Pollen preparation === | === Pollen preparation === | ||
Pollen was prepared by staining with fuchsin solution and embedding in Kaiser glycerin gelatin. Beginning with slide 09-100 I use the prestained (0. | Pollen was prepared by staining with fuchsin solution and embedding in Kaiser glycerin gelatin. Beginning with slide 09-100 I use the prestained (0.007% fuchsin, Kaise) glycerol jelly. Melting of the gelatin by means of a USB Cup Warmer Pearl, Model 834. The coverslip was then sealed with nail polish. Degreasing of the pollen with a few drops of ether and subsequent aspiration of the liquid with filter paper. | ||
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[[File:Tassenwärmer.jpg|200px]] | [[File:Tassenwärmer.jpg|200px]] | ||
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==== Glycerol gelatin (Kaiser) ==== | ==== Glycerol gelatin (Kaiser) ==== | ||
Put 7 g of gelatin (Gelatina alba Golddruck), 42 g of water, 50 g of glycerol 85% and 0.5 g of phenol in a 250 ml laboratory plastic bottle and keep it in a water bath at about | Put 7 g of gelatin (Gelatina alba Golddruck), 42 g of water, 50 g of glycerol 85% and 0.5 g of phenol in a 250 ml laboratory plastic bottle and keep it in a water bath at about 60°C. Shake in between. If everything has dissolved, keep the bottle in the water bath until all air bubbles have accumulated on the surface. Turn the bottle slowly and fill some small 30 ml bottles with the hot solution. Allow to cool. | ||
==== | ==== Glycerol gelatin (Kaiser) stained with fuchsine (0.007%)<small>[this staining method starts with slide 09-100]</small>==== | ||
14 g of gelatin (Gelatina alba Golddruck), 84 g of water, 100 g of glycerol 85%, 0.5 g of phenol and 14 mg fuchsine (C.I. 42510, z.B. Merck 1.15937)<br> | 14 g of gelatin (Gelatina alba Golddruck), 84 g of water, 100 g of glycerol 85%, 0.5 g of phenol and 14 mg fuchsine (C.I. 42510, z.B. Merck 1.15937)<br> | ||
==== Glycerol gelatin (Kisser) ==== | ==== Glycerol gelatin (Kisser) ==== | ||
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==== Chloralhydrate-solution ==== | ==== Chloralhydrate-solution ==== | ||
Dissolve 16 g of chloralhydrate in 10 g of water and add 5 ml of glycerol 85%. | Dissolve 16 g of chloralhydrate in 10 g of water and add 5 ml of glycerol 85%. | ||
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